Cymoplive is a platform that allows us to study cells in action and how they smartly tackle obstacles in our body. Cell movement or motility governs how cells migrate, invade, repair injuries, and develop tissues and organs. These processes are critical for a healthy, functioning body. Cell plasticity, in the form of individual or collective cell migration, has long-standing consequences and can help identify diseases and how much they have progressed. In the Cymoplive platform, cells are placed in a 3D maze where they need to overcome designed obstacles as they move through the maze. Cymoplive has a live-imaging friendly format compatible with all optical microscopes. The complexity of the maze can be customized based on the experimental setup and what is to be studied.